If a tube breaks in the centrifuge, what is the first step you should take?

When a tube breaks in the centrifuge, the correct initial response is to ensure safety and contain the situation. The primary action taken should be to discard the sleeve containing the broken tube and remove the opposing sleeve to prevent any potential hazards, such as exposure to biological materials or chemicals. By doing this, you reduce the risk of spreading any contaminants and maintain a safer working environment.

Collecting the broken tube in a safe manner, while also following proper protocols for hazardous waste, is essential in the event of a breakage. This approach addresses potential exposure before any other steps are considered, like turning off equipment or calling for assistance. Prioritizing the containment of the spill is crucial in handling such an incident effectively.

Other responses, such as turning off the centrifuge without addressing the immediate hazard or sealing the broken tube with tape, do not adequately manage the risk or ensure safety in the laboratory. Continuing to run the centrifuge could worsen the situation, posing further risks to personnel and the integrity of the samples. Therefore, the action of immediately discarding and removing the broken tube is the most appropriate first step in this scenario.